La vitamina D3 estimula el efecto anabólico de la leucina

Foro general ciencia, medicina, nutrición, salud pública, política

Moderador: Fisio

La vitamina D3 estimula el efecto anabólico de la leucina

Notapor culturismonovel » Jue, 19 Sep 2013, 15:39

La vitamina D3 potencia efecto en leucina e insulina en la síntesis proteica a través de mTOR y Akt.

SCOPE:

In recent years, there has been a growing body of evidence pointing to an effect of vitamin D on muscle mass and function. Our aim was to investigate the combined effect of 1,25(OH)2 -vitamin D3 (1,25(OH)2 D3 ) with anabolic factors insulin and leucine on protein fractional synthesis rate (FSR) and regulation in the mouse C2C12 myotube.

METHODS AND RESULTS:

After differentiation, myotubes were cultured in 1,25(OH)2 D3 solutions at 0, 1, or 10 nM for 72 h. Cells were treated by l-[1-13 C]valine and puromycin in presence or not of leucine and insulin, and protein FSR was determined by measuring tracer enrichments and puromycin incorporation in proteins, respectively. Protein expression and phosphorylation state of insulin receptor (IR), Akt, GSK3, mTOR, p70 S6 kinase, rpS6, and 4EBP1 were measured by Western blot. Transcript levels of IR and 1,25(OH)2 D3 receptor (VDR) were determined by qPCR. 1,25(OH)2 D3 (10 nM) with leucine and insulin increased protein FSR in C2C12 myotubes (14-16%). IR and VDR mRNA expression was increased with 1,25(OH)2 D3 treatment. The Akt/mTOR-dependent pathway was activated by insulin and leucine and further enhanced by 1,25(OH)2 D3 .

CONCLUSION:

1,25(OH)2 D3 sensitizes the Akt/mTOR-dependant pathway to the stimulating effect of leucine and insulin, resulting in a further activation of protein synthesis in murine C2C12 skeletal myotubes.


Lo único que no me gusta un pelo es que el estudio está financiado por Danone.

Pubmed: http://www.ncbi.nlm.nih.gov/pubmed/23929734
Organiza tu mente, haz deporte, come saludable, y sé feliz.
Avatar de Usuario
culturismonovel
 
Mensajes: 73
Registrado: Jue, 19 Sep 2013, 15:25
Ubicación: Temple Gym (Birmingham, UK)

Re: La vitamina D3 estimula el efecto anabólico de la leucina

Notapor Fisio » Jue, 19 Sep 2013, 17:57

No tiene por que ser un problema, Danone no tiene mucho interés en la conclusión de este estudio. Si fuera un estudio sobre niños o mayores y la osteoporosis ya mosquearía bastante más, pero este estudio no les da ningún titular.

Lo movemos a nutrición deportiva
Avatar de Usuario
Fisio
Administrador del Sitio
 
Mensajes: 4662
Registrado: Dom, 01 Sep 2013, 14:18


Volver a Muscleblog

¿Quién está conectado?

Usuarios navegando por este Foro: No hay usuarios registrados visitando el Foro y 2 invitados